Commentary: An excellent review and the principal reference for this alignment is Moller JV, Juul B, le Maire M 1996 Biochim Biophys Acta 1286:1-51.. Topography of P-type pumps:

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1-250 N-terminal sequence: cytoplasmic orientation; few conserved motifs, even among isoforms. S1: M1: 114-128 L1-2: M2: 142-162 S2 L2-3: B = small cytoplasmic domain: proteolysis/mutations in this region prevent E1->E2.

Putative cation-binding sites: in CaSR1 index(res#) seg seq 368(309) M4 PEG 846(771) M5 GEV 871(796) M6 NLV TD 989(908) M8 IEM

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251-500 S3 M3: 314-337 L3-4 M4: 347-366 S4 L4-5: C = large cytoplasmic domain; catalytic phosphorylation site.

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501-750

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751-1000
• * J= junctional or "hinge" region (gray)
• S5
• M5: 835-858
• L5-6
• M6: 862-884
• L6-7
• M7: 912-934
• L7-8
• M8: 977-997
• L8-9

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1000- M9: 1011-1037 L9-10 M10: 1049-1067 C-term