Background Subtraction and Image Normalization

Author: Jeffrey Kuhn (jkuhn at ccwf.cc.utexas.edu)
The University of Texas at Austin
Ben Dubin-Thaler (bjd14 at columbia.edu)
Columbia University
History: 2000/08/22: First version
2006/02/16: Works with stacks
Limitations:Currently limited to 16-bit images
Source: Normalize_.java
API: Normalize_.html
Installation: Download Normalize_.class to the plugins folder and restart ImageJ.
Description: This plugin simplifies the task of background subtraction and image normalization given a brightfield image and/or a background image. This plugin is currently limited to 16-bit images, but could easily be extended to 8-bit, 32-bit, or even color images.

Possible normalization methods:

If both BRIGHT and DARK images are specified

IMAGE = scale * (IMAGE - DARK) / (BRIGHT - DARK)
If only BRIGHT image is specified
IMAGE = scale * IMAGE / BRIGHT
If only DARK image is specified

IMAGE = IMAGE - DARK

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