C. Cameras and Light Boxes
Quantitative image analysis using Image
requires the use of a high quality TV camera and light source. You should select
a high resolution solid-state (CCD) monochrome camera designed for imaging applications.
There should be a way to disable any automatic gain control (AGC) or automatic black
level features. The light source should provide uniform illumination, as well as stable
output with normal line voltage variation.
One CCD camera that is well suited for quantitative densitometry is the Sony XC-77.
Other CCD cameras to consider include the Sierra Scientific MS-4030, the COHU Model
4815-5000, and the Dage CCD-72. Sierra Scientific, COHU and Dage also sell lenses
and filters. When ordering a camera, particularly the Sony XC-77, make sure it comes with
all necessary components, such as power supply, tripod attachment (if needed), and
Imaging Research, creator of the highly regarded MCID biomedical image analysis system
for the IBM-PC, makes a stabilized fluorescent light box that provides the uniform
illumination needed for autoradiographic densitometry. They also sell several needed
accessories, including the Nikon Micro-Nikkor f2.8/55 mm lens, the F-C adapter needed
to connect the lens to a TV camera, and the Kaiser RS-1 copy stand. In addition,
they sell Sony and Dage cameras.
For digitizing gels, Fotodyne has an unstabilized light box called the VariQuest 100
that is considerable less expensive than the one from Imaging Research. Fotodyne
also distributes a program called "Collage" for imaging of electrophoresis gels,
blots and autoradiograms.
For specialized applications, Perceptics has a NuBus frame grabber card that supports
the 1340X1037 resolution Videk MegaPlus camera, and can supply a version of Image
that supports the MegaPlus. Photometrics has a cooled CCD imaging system for the
Macintosh that supports CCDs with a resolution of up to 2K X 2K. The Photometrics
system, however, is not directly supported by Image
. Dage-MTI has a real-time video processor (the DSP-200) that does real-time averaging
and integration to enhance noisy, low intensity images. They also have an image intensifier
for CCD cameras.
San Diego, California
fax: 615-966 9330
701 N. Roeske Ave.
Michigan City, Indiana 46360
Imaging Research Inc.
St. Catharines, Ontario
Canada L2S 3A1
Sony Corporation of America
Component Products Company
10833 Valley View St.
Cypress, CA 90630